Cultured human monocytes synthesize and secrete alpha2-macroglobulin.
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منابع مشابه
Cultured human monocytes synthesize and secrete alpha2-macroglobulin
alpha2-Macroglobulin levels in the supernates of cultures of different subpopulations of human peripheral blood mononuclear leukocytes were assayed by a radioimmunoassay. Unfractionated mononuclear leukocytes produced greater amounts of the macroglobulin (4.0 vs. 0.8 ng/10(6) cells) than did subpopulations enriched in T or B+T lymphocytes, by passage through nylon wool or cotton wool columns, r...
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Thrombospondin, one of the major glycoproteins released from a-granules of thrombin-stimulated platelets, is a disulfide-linked trimer of 1 60,000-dalton subunits. Cultured human monocytes secreted thrombospondin (determined by an enzyme-linked immunosorbent assay) into the culture medium in a time-dependent manner (1 .45 g/10’ cells/24 hr); secretion was totally blocked by cycloheximide (1 g/m...
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The following observations indicate that cultured human WI-38 fibroblasts synthesize and secrete alpha2-macroglobulin into serum-free medium: (a) after incubation of cultures with [35S]L-methionine, a labeled protein appeared in the medium which was precipitated by antiserum directed against alpha2-macroglobulin; (b) after incubation of cultures with [35S]L-methionine, a major band of radioacti...
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alpha2-Macroglobulin (alpha2M) has been identified on the luminal surface of endothelial cells in sections of normal human arteries, veins, and lymphatics by the indirect immunofluorescent technique. The specificity of the immunofluorescent reaction was confirmed by immunoabsorption studies. Prior absorption of the anti-alpha2M antiserum by purified alpha2M at equivalence completely inhibited e...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1977
ISSN: 0022-1007,1540-9538
DOI: 10.1084/jem.145.6.1580